Journal: Frontiers in Microbiology

Article Title: Modeling Metabolic Interactions in a Consortium of the Infant Gut Microbiome

doi: 10.3389/fmicb.2017.02507

Figure Lengend Snippet: Changes in bacterial population during growth on FOS, expressed as percentage of the co-culture in time. (A) experimental data of co-cultures; (B) model estimation of abundance in co-cultures; (C) abundance of the four-species co-culture in microplates, experimental (Left) and estimated by the model (Right); (D) abundance in the four-species co-culture in the bioreactor during growth on FOS, experimental (Left) and estimated by the model (Right).

Article Snippet: Volumes of 200 μL of inoculated mZMB were placed in 96 well sterile microplates, covered with 30 μL of sterile mineral oil, and incubated in anaerobic jars at 37°C for either 24, 48, or 72 h. In parallel, growth was monitored every 12 h in a microplate reader (Tecan Infinite M200 PRO, Switzerland).

Techniques: Co-Culture Assay

Journal: Frontiers in Microbiology

Article Title: Modeling Metabolic Interactions in a Consortium of the Infant Gut Microbiome

doi: 10.3389/fmicb.2017.02507

Figure Lengend Snippet: Heat map representing FOS concentration in co-cultures and its prediction by the model. (A) FOS concentration in paired co-cultures. (B) Model estimation of FOS concentration in paired co-cultures; (C) experimental and predicted FOS concentration in the four-species co-culture in microplates; (D) experimental and predicted FOS concentration in the four-species co-culture in the bioreactor.

Article Snippet: Volumes of 200 μL of inoculated mZMB were placed in 96 well sterile microplates, covered with 30 μL of sterile mineral oil, and incubated in anaerobic jars at 37°C for either 24, 48, or 72 h. In parallel, growth was monitored every 12 h in a microplate reader (Tecan Infinite M200 PRO, Switzerland).

Techniques: Concentration Assay, Co-Culture Assay